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dapi cell staining solution  (Beyotime)


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    Beyotime dapi cell staining solution
    Dapi Cell Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+c1002/DAPI/pm41745568-125-9-13
    Average 99 stars, based on 32584 article reviews
    dapi cell staining solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Double Staining:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Blocking Assay:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Red Blood Cell Lysis:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    CCK-8 Assay:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Staining:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Modification:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Recombinant:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
    Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..



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    Image Search Results


    Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Journal: Journal of Advanced Research

    Article Title: Increased Rab1a accelerates osteoarthritis by inhibiting autophagy via activation of the mTORC1-S6K pathway

    doi: 10.1016/j.jare.2024.11.009

    Figure Lengend Snippet: Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

    Article Snippet: Subsequently, The TUNEL reaction mixture was applied at room temperature for 1 h. This was followed by coloring the nuclei with DAPI Staining Solution(Beyotime, China).

    Techniques: Transfection, Control, Immunohistochemical staining, TUNEL Assay, Staining, Fluorescence, End Labeling