Double Staining:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Blocking Assay:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Red Blood Cell Lysis:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
CCK-8 Assay:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Staining:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Modification:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Recombinant:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
Enzyme-linked Immunosorbent Assay:Article Title: A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases
Article Snippet: Bovine serum albumin was purchased from Klon tech; mPEG-NH 2 (MW5000) was purchased from Shanghai seebio biotechnology Co., Ltd; All reagents were used without further purification. .. 100× Penicillin–Streptomycin–Gentamicin Solution (P1410), PBS, Trypsin–EDTA solution, 0.25% (without phenol red) (9002-07-7), Calcein AM/PI live/dead cell double staining kit (CA1630), 5% BSA Blocking Buffer (SW3015), Percoll Cell separation solution (65455-52-9), Red blood cell lysis buffer (R1010), Collagenase IV (9001-12-1), DNase I (9003-98-9) were purchased from Beijing Solarbio Science & Technology Co., Ltd; Cell counting kit-8 (Catalog No. K1018) was purchased from APExBIO technology Ltd.; DAPI staining solution (C1002) was purchased from Beyotime biotechnology; Annexin V-FITC/PI apoptosis detection kit (40302ES50) was purchased from Shanghai Yisheng biotechnology Co., Ltd.; Fetal bovine serum (11011-8611) was purchased from Zhejiang tianhang biology Co., Ltd; Dulbecco's modified eagle medium (30030), Roswell Park Memorial Institute (RPMI) 1640 medium, CD11b monoclonal antibody (M1/70), APC (17-0112-82), FOXP3 monoclonal antibody (3G3), alexa fluor 647 (MA5-18160), CD11c monoclonal antibody (N418), FITC (11-0114-85), CD80 (B7-1) monoclonal antibody (16-10A1), APC (17-0801-82), CD86 (B7-2) monoclonal antibody (GL1), PE (12-0862-82), CD3 monoclonal antibody (17A2), APC (17-0032-80), CD8a monoclonal antibody (53–6.7), PE (12-0081-85), CD4 monoclonal antibody (GK1.5), FITC (11-0041-81), CD45 monoclonal antibody (30-F11), FITC (11-0451-82), F4/80 monoclonal antibody (BM8), APC (17-4801-80) were purchased from eBioscienceTM; APC anti-mouse CD86 (105011), PE anti-mouse CD206 (MMR) (141705), Recombinant mouse IL-4 (carrier-free) (574302) were purchased from BioLegend; Calreticulin polyclonal antibody (27298-1-AP) was purchased from Proteintech; TNF-α, IFN-γ, IL-6, IL-2 ELISA kits (EK202/2-48) were purchased from Multisciences (Lianke) Biotech, Co., Ltd. ..
|